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rspa-cfast  (Addgene inc)


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  • 90

    Structured Review

    Addgene inc rspa-cfast
    Rspa Cfast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rspa-cfast/rspa+cfast/pmc11557831-390-33-42
    Average 90 stars, based on 1 article reviews
    rspa-cfast - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Simultaneous detection of membrane contact dynamics and associated Ca 2+ signals by reversible chemogenetic reporters.
    Article Snippet: For the calculation of the genuine change of fluorescence of the different MCS splitFASTbased probes, we performed parallel experiments for each experimental condition/stimulus in cells expressing either cytosolic RspANFAST (plasmid pAG573 FRB-RspA(N)-IRES-mTurquoise2)15 and RspA-CFAST (plasmid pAG580 FKBP-RspA(C)-IRES-iRFP670)15, or NFAST (pAG148 FRB-NFAST, Addgene #130812) and CFAST10 (pAG241 FKBP-CFAST10, Addgene #130814), keeping the same illumination/exposure parameters.

    Article Title: Simultaneous detection of membrane contact dynamics and associated Ca 2+ signals by reversible chemogenetic reporters
    Article Snippet: For the calculation of the genuine change of fluorescence of the different MCS splitFAST-based probes, we performed parallel experiments for each experimental condition/stimulus in cells expressing either cytosolic RspA-NFAST (plasmid pAG573 FRB-RspA(N)-IRES-mTurquoise2) and RspA-CFAST (plasmid pAG580 FKBP-RspA(C)-IRES-iRFP670) , or NFAST (pAG148 FRB-NFAST, Addgene #130812) and CFAST10 (pAG241 FKBP-CFAST10, Addgene #130814), keeping the same illumination/exposure parameters.

    Expressing:

    Article Title: Simultaneous detection of membrane contact dynamics and associated Ca 2+ signals by reversible chemogenetic reporters.
    Article Snippet: For the calculation of the genuine change of fluorescence of the different MCS splitFASTbased probes, we performed parallel experiments for each experimental condition/stimulus in cells expressing either cytosolic RspANFAST (plasmid pAG573 FRB-RspA(N)-IRES-mTurquoise2)15 and RspA-CFAST (plasmid pAG580 FKBP-RspA(C)-IRES-iRFP670)15, or NFAST (pAG148 FRB-NFAST, Addgene #130812) and CFAST10 (pAG241 FKBP-CFAST10, Addgene #130814), keeping the same illumination/exposure parameters.

    Article Title: Simultaneous detection of membrane contact dynamics and associated Ca 2+ signals by reversible chemogenetic reporters
    Article Snippet: For the calculation of the genuine change of fluorescence of the different MCS splitFAST-based probes, we performed parallel experiments for each experimental condition/stimulus in cells expressing either cytosolic RspA-NFAST (plasmid pAG573 FRB-RspA(N)-IRES-mTurquoise2) and RspA-CFAST (plasmid pAG580 FKBP-RspA(C)-IRES-iRFP670) , or NFAST (pAG148 FRB-NFAST, Addgene #130812) and CFAST10 (pAG241 FKBP-CFAST10, Addgene #130814), keeping the same illumination/exposure parameters.

    Plasmid Preparation:

    Article Title: Simultaneous detection of membrane contact dynamics and associated Ca 2+ signals by reversible chemogenetic reporters.
    Article Snippet: For the calculation of the genuine change of fluorescence of the different MCS splitFASTbased probes, we performed parallel experiments for each experimental condition/stimulus in cells expressing either cytosolic RspANFAST (plasmid pAG573 FRB-RspA(N)-IRES-mTurquoise2)15 and RspA-CFAST (plasmid pAG580 FKBP-RspA(C)-IRES-iRFP670)15, or NFAST (pAG148 FRB-NFAST, Addgene #130812) and CFAST10 (pAG241 FKBP-CFAST10, Addgene #130814), keeping the same illumination/exposure parameters.

    Article Title: Simultaneous detection of membrane contact dynamics and associated Ca 2+ signals by reversible chemogenetic reporters
    Article Snippet: For the calculation of the genuine change of fluorescence of the different MCS splitFAST-based probes, we performed parallel experiments for each experimental condition/stimulus in cells expressing either cytosolic RspA-NFAST (plasmid pAG573 FRB-RspA(N)-IRES-mTurquoise2) and RspA-CFAST (plasmid pAG580 FKBP-RspA(C)-IRES-iRFP670) , or NFAST (pAG148 FRB-NFAST, Addgene #130812) and CFAST10 (pAG241 FKBP-CFAST10, Addgene #130814), keeping the same illumination/exposure parameters.



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